Optimization conditions for extracting polysaccharide from Angelica sinensis and its antioxidant activities
In this study, polysaccharides from Angelica sinensis were extracted using the ultrasound-assisted extraction method. Based on the results of single factor experiments and orthogonal tests, three independent variables—water/raw material ratio, ultrasound time, and ultrasound power—were selected for investigation. Then, we used response surface methodology to optimize the extraction conditions. The experimental data were fitted to a quadratic equation using multiple regression analysis, and the optimal conditions were as follows: water/raw material ratio, 43.31 mL/g; ultrasonic time, 28.06 minutes; power, 396.83 W. Under such conditions, the polysaccharide yield was 21.89 ± 0.21%, which was well matched with the predicted yield. In vitro assays, scavenging activity of superoxide anion radicals, hydroxyl radicals, and 2,2-diphenyl-1-picry-hydrazyl radical showed that polysaccharides had certain antioxidant activities and that hydroxyl radicals have a remarkable scavenging capability. Therefore, these studies provide reference for further research and rational development of A. sinensis polysaccharide. © 2016
Tian, S.; Hao, C.; Xu, G.; Yang, J.; and Sun, R.
"Optimization conditions for extracting polysaccharide from Angelica sinensis and its antioxidant activities,"
Journal of Food and Drug Analysis: Vol. 25
, Article 27.
Available at: https://doi.org/10.1016/j.jfda.2016.08.012
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.