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Article Title

Determination of flavonoids in Daphnis Genkwae Flos by high performance liquid chromatography

Abstract

In order to evaluate the quality of Daphnis Genkwae Flos, a high performance liquid chromatographic method (HPLC) was developed. Six flavonoid constituents from Daphnis Genkwae Flos, genkwanin 5-O-β-D-primeveroside (1), genkwanin 5-O-β-D-glucoside (2), genkwanin (3), potassium apigenin 7-O-β-D-glucuronate (4), apigenin (5) and tiliroside (6) determined by this simple, rapid and accurate method. Samples were analyzed using a Cosmosil 5C18-AR reversed phase column by gradient elution with varied proportion of 1.0 % (v/v) acetic acid and acetonitrile as mobile phase at 254 nm. Sulfamethoxypyridazine was used as the internal standard. Regression equations revealed the linear relationship (correlation coefficients: 0.9994∼0.9999) between the peak-area ratios flavonoid to internal standard and concentrations of flavonoids. The recoveries of six flavonoids were between 100.9∼106.1%. The relative standard deviations of six flavonoids ranged between 0.49∼3.46% (intraday) and 0.52∼4.61% (interday). The contents of the six flavonoids in sixteen crude drugs of Daphnis Genkwae Flos were 1 0.13∼0.40%, 2 0.02∼0.12%, 3 0.24∼0.56%, 4 0.16∼1.19%, 5 0.30∼0.73% and 6 0.15∼0.57%, respectively.

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