Article Title

Cimifugin Preparation and Quantitative Analysis of Saposhnikoviae Radix by HPLC


Cimifugin, one of the principal analgesic and anti-hypertensive component of Fang-Feng, was isolated from the root of Saposhnikovia divaricata. Reversed-phase high-performance liquid chromatography was employed to determine the contents of cimifugin in Fang-Feng. The separation was performed on a LiChrospher 100 RP-18e column (4 × 250 mm) by isocratic elution with acetonitrile-water (18:82) as the mobile phase at a flow-rate of 1.0 mL/min, the temperature being kept at 40°C and the detection set at 220 nm. Methylparaben was used as the internal standard. The regression equation revealed linear relationship between the peak-area ratio (marker substance/internal standard) and concentration. We found that Fang-Feng extracted with twenty-fold of 70% aqueous methanol under sonication for 40 minutes gives the best yield of cimifugin. The contents of cimifugin in commercially available Saposhnikoviae Radices have been determined and the amount was found to vary from 2.66 × 10-2% to 0.19 × 10-2%. This HPLC method can rapidly and easily quantify the cimifugin present in Fang-Feng and can be used for quality control of this commercial crude drug.

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