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Article Title

Solid-phase extraction and high performance liquid chromatographic determination of steroids adulterated in traditional Chinese medicines

Abstract

A systematic evaluation and comparison of the recoveries of eight steroids from spiked traditional Chinese medicine (TCM) extract using silica gel solid-phase extraction (SPE) are reported. The eight steroids, betamethasone, cortisone acetate, dexamethasone, hydrocortisone acetate, methylprednisolone, prednisolone, prednisone and triamcinolone, adulterated in TCM were assayed simultaneously by high performance liquid chromatography on a reverse phase column. This chromatographic method was carried out using isocratic elution with acetonitrile and water (3:7). Fludrocortisone acetate was used as an internal standard and detection was at 240 nm. Calibration curves of the eight adulterants were constructed in the range 6-96 μg/mL. Their correlation coefficients ranged from 0.9983 to 0.9996. The relative standard deviations of the eight steroids for intraday and interday analyses were 0.7-4.1% and 1.0-4.4%, respectively. Optimized experimental conditions including solvent composition, TCM extract concentration and different brands of SPE cartridge were also investigated.

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