Amplification of the region of 16S-23S rRNA spacer was performed on 9 isolates of Mycobacterium bovis BCG (including Brazilian, Connaught, Danish, Glaxo, Japanese, Pasteur, Russian, Taiwan and Tice) and one isolate of Mycobacterium smegmatis using BCGF1 and BCGR1 as the primer pair. In this study we found 350-bp of PCR product in all the BCG strains but a 456-bp amplified fragment from M. smegmatis. Consequently, this primer pair may be a potential tool in differentiating between these two Mycobacterium species.
Kuan, C.-P. and Lin, C.-P.
"Rapid Differentiation of BCG Vaccine from M. smegmatis by Genomic Amplification,"
Journal of Food and Drug Analysis: Vol. 7
, Article 2.
Available at: https://doi.org/10.38212/2224-6614.2872