High Performance Liquid Chromatographic Determination of Anthocyanin Pigments in Some Varieties of Black Rice
Anthocyanin pigments in ten pigmented rice cultivars were isolated with cellulose thin-layer chromatography and preparative high performance liquid chromatography (HPLC), and further identified with conventional chromatographic techniques. Individual anthocyanins were then quantified by HPLC using a reversed ODS-5 column and detected at 280 nm. Ten rice varieties contained two major anthocyanins, cyanidin 3-glucoside and peonidin 3-glucoside. Total anthocyanin contents varied greatly in the range of 0-493 mg/100 g grain among varieties. Cyanidin 3-glucoside, an agent having important oxygen radical absorbing capacity (ORAC), was most abundant in Suwon #415 variety (470 mg/100 g grain), while Jawangdo (10 mg/100 g grain) and Ilpumbyeo (not detected) had the smallest amount of anthocyanin among the samples examined. As for the distribution ratio of individual anthocyanin, Suwon #415 was characterized by a relatively high percentage of cyanidin 3-glucoside (95.3%); whereas, Suwon #425 and Heugjinmi were characterized by a relatively larger amount of peonidin 3-glucoside.
Ryu, S.N.; Park, S.Z.; and Ho, C.-T.
"High Performance Liquid Chromatographic Determination of Anthocyanin Pigments in Some Varieties of Black Rice,"
Journal of Food and Drug Analysis: Vol. 6
, Article 6.
Available at: https://doi.org/10.38212/2224-6614.2893