Techniques for detecting organosphosphate pesticides residues in aquaria


For detecting residue of organosphosphorus pesticides (OPs) in polluted aquaria, a suitable multiresidue analyses method is necessary. The fate of pesticides in pond water must be understand, as well. In this study, one major purpose was to screen suitable pre-treatment methods of water/fish sampling for multiresidue analyses of OPs in aquaria. The second purpose was to study the distribution of pesticides from water to fish. To analyze water samples, the pretreatment method of Li (1984) and Okumura and Nishikawa (1995) were compared. From the results with 8 OPs, the latter is beneficial for analyzing multiresidues of OPs in aquaria for recoveries with fewer coefficients of variation and less time cost. To analyze fish samples, a method that used a safe solvent and one that detected the pesticides mostly at same time was developed. This method is suitable for OPs that have low polarity. Cyprinus carpio was exposed to three commercial OPs, including chlorpyrifos 40.8% EC, EPN 45% EC and fenitrothion 50 % EC, to evaluate the distribution of pesticides in fish tissue from water. The pesticide was applied as a single dose of their LC50-96hrs to Cyprinus carpio for 48hrs. Data of pesticides residue in fish muscle showed that these pesticides can be bioconcentrated to fish body within a short time. The necessity of analyzing residues in fish to determine if an aquarium is polluted by OPs or not is discussed in this paper.

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