A new method for determining heme iron in pork using solid phase extraction and graphite furnace atomic absorption spectrophotometer
A new method for selective determination of heme iron in pork was developed. First heme iron in pork was extracted with 80% acetone acidified with 2% hydrochloric acid to below pH 1.0. After adjusting the concentration of acetone to 40%, the solution was passed through a Sep-Pak C 18 cartridge. Heme was adsorbed on the cartridge, and was then eluted with 80% acetone. The eluent was analyzed for heme iron with a graphite furnace atomic absorption spectrophotometer. Recovery studies were performed by adding 1.75, 2.50 and 3.75 μg HFe/g myoglobin and 3.00, 7.00 and 10.00 μg HFe/g hemoglobin into the pork. Recoveries were in the range of 97.9 ∼ 99.3%.
Chen, S.-S.; Chang, W.-H.; and Chou, S.-S.
"A new method for determining heme iron in pork using solid phase extraction and graphite furnace atomic absorption spectrophotometer,"
Journal of Food and Drug Analysis: Vol. 6
, Article 1.
Available at: https://doi.org/10.38212/2224-6614.2904