Article Title

Application of capillary electrophoresis for identification of the authenticity of bird's nests


A method using capillary electrophoresis (CE) to determine amino acid prfile was developed to identify the authenticity of bird's nest. Bird's nest samples were hydrolyzed in acid and alkali, respectively. The protein hydrolysates were then derivatized with dansyl chloride, and analyzed by CE. The average recovery of amino acids was 102.30%. The detection limits were between 0.01 and 0.03 pmole. Ten bird's nest samples and twenty bird's nest in rock sugar syrup were analyzed. The bird's nest samples were found to contain 47.05-58.91% crude protein, while bird's nest in rock sugar syrup were found to contain 1.44-58.45%. All the bird's nest samples were identified to be authentic based on amino acid profile matching. Among 20 bird's nest in rock sugar syrup, seven were considered to be substituted with agar, natural gum or pork skin. CE analysis of amino acid profile was found to be an efficient and sensitive technique for identifying the authenticity of bird's nests.

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