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Article Title

Determination of sennosides A and B in diet tea by HPLC

Abstract

A rapid high performance liquid Chromatographic method was developed for determination of sennosides A and B in senna leaves, pods and diet tea. The determination was carried out by using a gradient elution with acetonitrile and 1% acetic acid on an Inertsil ODS-2 column. The linear calibration ranges of sennosides A and B were 7.52-37.60 μg/ml (r=0.9998) and 8.32-49.92 μ/ml (r=0.9999), respectively. The recoveries of sennosides A and B were almost quantitative from the senna leaves and pods, respectively. The relative standard deviations of sennosides A and B for intraday and interday analyses were 0.24-1.72% and 0.23-1.71%, respectively. The contents of sennosides A and B in leaves and pods of Cassia angustifolia Vahl have been determined and the results were as follows: sennoside A 0.64,1.46% and sennoside B 1.57, 2.30%. The contents of sennosides A and B in seven diet tea samples have been determined and the results were as follows: sennoside A 0.10-0.19% and sennoside B 0.16-0.28%.

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