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Article Title

The enterotoxigenic types for 62 Staphylococcus aureus isolates from raw milk and some other animal specimens

Abstract

A commercial ELISA kit (RIDASCREEN® SET A, B, C, D, E. R-Biopharm GmbH, Darmstadt, Germany) was used to examine the enterotoxigenecity of 76 Staphylococcus aureus strains. Of these strains, 62 were isolated from raw milk, 3 from whale, 4 from dolphin, 2 from cow milk with mastitis, 2 from pig, and 1 each from chicken, mouse, and dog. Type A, B, and C enterotoxins were detected in 26, 2, and 1 strains, respectively. Neither type D nor type E enterotoxin-producing strain was observed. In addition, DNA amplification by capillary polymerase chain reaction (PCR) with specific primers was used to detect the presence of enterotoxin A gene. The results showed that 26 strains harbored this gene. Study on the detection sensitivity of PCR indicated that the lowest cell number required for positive reaction was 10 4 CFU/ml.

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