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Article Title

Determination of aflatoxin M1 in milk and milk powder using immuno-affinity column and fluorescence measurement

Authors

Y.-M. Fu

Abstract

The method using immuno-affinity column clean-up and fluorescence measurement was evaluated for detection of aflatoxin M1 in milk and milk powder. Two kinds of commercial immuno-affinity columns used were Afla Test-P Column and AFLAPREP M. The results are shown below : (1) By using Afla Test-P Column and Fluorometer, the average recoveries of aflatoxin M1 from defatted milk spiked at the levels of 0.5 and 1.0ppb were 84.4±7.0% and 94.2±9.5%. The detection limit was 0.1ppb. This method is easy and suitable for multi-sample treatment. (2) By using AFLAPREP M, the average recoveries of aflatoxin M1 from defatted milk spiked at the levels of 0.1, 0.5 and 1.0ppb were 84.7±2.7 %, 88.7±1.9%and 89.2±4.2%. The detection limit was 0.05ppb. This method is more sensitive and can be used at the laboratory equipped with HPLC. (3) 25 samples of milk and 25 samples of milk powder were perchased from supermarkets in Taipei from July 1994 to June 1995 and tested by AFLAPREP M. Aflatoxin M1 was detected in 3 samples of milk. The toxin contents were 0.33, 0.12, 0.08ppb respectively. Milk powders were reconstituted and tested, no aflatoxin M1 was detected in 25 samples.

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