Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10-1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.
Quan, Shuai; Ji, Kaixiang; Liu, Fangshuo; Barkae, Tesfaye Hailemariam; Halawa, Mohamed Ibrahim; Hanif, Saima; Lou, Baohua; Li, Jianping; and Xu, Guobao
"Chemiluminescence of lucigenin-tetracycline and itsapplication for sensitive determination of procyanidin,"
Journal of Food and Drug Analysis: Vol. 30
, Article 9.
Available at: https://doi.org/10.38212/2224-6614.3411
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