Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%–116.32%), precision (covariance < 20%), sensitivity (LOD < 0.1 µg/mL), turnaround time (< 5 minutes), and linearity (R2 = 0.9827–0.9992) within 0.1–100 µg/mL.
Su, Hung; Lin, Hsin-Hua; Su, Lin-Jhen; Lin, Chia-Chen; Jiang, Zong-Han; Chen, Shiang-Jin; Shiea, Jentaie; and Lee, Chi-Wei
"Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics,"
Journal of Food and Drug Analysis: Vol. 30
, Article 3.
Available at: https://doi.org/10.38212/2224-6614.3399
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