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Article Title

Screening chemical drugs used to adulterate in rheumatic and analgesic traditional Chinese medicine by HPLC-DAD

Abstract

High-performance liquid chromatography with photodiode array detector (DAD) was developed for the identification of adulterants in rheumatic and analgesic Chinese medicine. A study was performed by using a gradient glution with acetonitrile and 0.1% acetic acid solution on a C-18 column. This method was applied to simultaneously screen 17 synthetic drugs adulterated in traditional Chinese medicine within half an hour. The drugs belonged to six pharmacological categories: antipyretic analgesics, glucocorticoids, diuretics, CNS stimulants, muscle relaxants and sedatives. They included acetaminophen, aminopyrine, bucetin, ethoxybenzamide, indomethacin, ketoprofen, mefenamic acid, phenylbutazone, piroxicam, salicylamide, dexamethasone, prednisolone, chlormezanone, chlorzoxazone, hydrochlorothiazide, diazepam and caffeine. This method can provide higher resolution and greater efficiency than thin-layer chromatography for screening adulterated synthetic drugs. Three traditional Chinese medicines sold by the dealer of Chinese natural drugs that were collected by the consumer centers of local health bureaus from August, 1993 to September, 1993 and all were examined by this method. Five synthetic drugs, mefenamic acid, hydrochlorothiazide, chlorzoxazone diazepam and caffeine, four synthetic drugs, mefenamic acid, indomethacin, salicylamide and hydrochlorothiazide, and seven synthetic drugs, acetaminophen, indomethacin, ethoxybenzamide, hydrochlorothiazide, chlorozoxazone, diazepam and caffeine were found from Sample A, B and C, respectively.

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