Variations in bovine milk oligosaccharides after calving using p-aminobenzoic ethyl ester closed-ring labeling and negative ion electrospray LC/MS/MS

Authors

Han-Ju Chien, Institute of Molecular Biology, National Chung Hsing University, Taichung, TaiwanFollow
Yi-Chun Ma, Department of Chemistry, National Taiwan University, Taipei, TaiwanFollow
Yi-Feng Zheng, Institute of Molecular Biology, National Chung Hsing University, Taichung, TaiwanFollow
Cheng-Yu Kuo, Institute of Molecular Biology, National Chung Hsing University, Taichung, TaiwanFollow
Wei-Chen Wang, Institute of Molecular Biology, National Chung Hsing University, Taichung, TaiwanFollow
Guor-Rong Her, Department of Chemistry, National Taiwan University, Taipei, Taiwan
Chien-Chen Lai, Institute of Molecular Biology, National Chung Hsing University, Taichung, TaiwanFollow

Abstract

A strategy was proposed to analyze bovine milk oligosaccharides using p-aminobenzoic ethyl ester (ABEE) closed-ring labeling and C18 capillary liquid chromatography negative ion electrospray tandem mass spectrometry. Linkage specific fragment ions were used to identify oligosaccharide isomers. By constructing the mass chromatograms using linkage specific fragment ions, isomers were differentiated based on m/z values as well as temporal separation provided by liquid chromatography. In addition to disialyllactose and the single isomer lacto-N-neohexaose, four pairs of linkage isomers including 3’/6’-sialyllactose (3’/6’-SL), 3’/6’-sialyllactosamine (3’/6’-SLN), 3’/6’-sialylgalactosyl-lactose (3’/6’-SGL), and lacto-N-tetraose/lacto-N-neotetraose (LNT/LNnT) in bovine milk were investigated. Variations of bovine milk oligosaccharides in a lactation period of 72 hrs after calving were studied. Sialylated oligosaccharide was found to be distinctively more abundant in milk of the first 24 hrs, decreasing in successive milkings. For the first time, the variation of lacto-N-tetraose in bovine milk was reported.

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Recommended Citation

Chien, Han-Ju; Ma, Yi-Chun; Zheng, Yi-Feng; Kuo, Cheng-Yu; Wang, Wei-Chen; Her, Guor-Rong; and Lai, Chien-Chen (2021) "Variations in bovine milk oligosaccharides after calving using p-aminobenzoic ethyl ester closed-ring labeling and negative ion electrospray LC/MS/MS," Journal of Food and Drug Analysis: Vol. 29 : Iss. 4 , Article 15.
Available at: https://doi.org/10.38212/2224-6614.3386

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