A gas chromatography–tandem mass spectrometry (GC–MS/MS) method was developed for the simultaneous detection and quantification of five phytogenic compounds (carvone, menthol, thymol, carvacrol and methyl salicylate) in chicken breast. Chicken breast samples were analyzed using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) approach using acetonitrile as extraction solvent, followed by a d-SPE (dispersive-solid phase extraction) clean up step. The linearities for the 5 substances were studied in the range between 2 and 100 µg/L and the coefficients of determination (R2) were always > 0.995. Matrix effects were also assessed by comparing the slopes obtained in solvent and chicken breast matrix. The recoveries for all the substances at 3 different spike levels (5, 10 and 50 µg/kg) were in the range 80-102% with RSDs < 15%. The instrumental limits of quantification were in the range 2.7-4.8 µg/kg, while the reporting level of the method was 5 µg/kg for all the aforementioned compounds. The method was successfully applied to 10 chicken breasts samples from the local market.
Cozzi, Federico; Eder-Neuhauser, Irene; Teichmann, Klaus; and Zaunschirm, Mathias
"Determination of Phytochemical Compounds in Chicken Breast by Gas Chromatography-tandem mass spectrometry,"
Journal of Food and Drug Analysis: Vol. 29
, Article 5.
Available at: https://doi.org/10.38212/2224-6614.3288
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