Anti-inflammatory effects of peptides from a marine algicolous fungus Acremonium sp. NTU492 in BV-2 microglial cells

Authors

George Hsiao, Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, TaiwanFollow
Shih-Wei Wang, Department of Medicine, Mackay Medical College, New Taipei City, TaiwanFollow
Yin-Ru Chiang, Biodiversity Research Center, Academia Sinica, Taipei, TaiwanFollow
Wei-Chiung Chi, Institute of Food Science, National Quemoy University, Kinmen, TaiwanFollow
Yueh-Hsiung Kuo, Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, TaiwanFollow
Do Anh Phong, International Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei, TaiwanFollow
Chia-Yu Chen, Institute of Fisheries Science, National Taiwan University, Taipei, TaiwanFollow
Tzong-Huei Lee, Institute of Fisheries Science, National Taiwan University, Taipei, TaiwanFollow

Abstract

Located in tropical and subtropical region, Taiwan is an island with high algal species diversity. In this study, a number of fungal strains were isolated from marine macroalgae collected from northeastern intertidal zone of Taiwan. Preliminary anti-inflammatory screening has shown that the methanolic extracts of solid fermented products of the red alga Mastophora rosea-derived fungal strain Acremonium sp. NTU492 exhibited significant bioactivity. In an attempt to disclose the active principles from this fungal strain, a series of separation and purification was thus undertaken, which has led to the isolation and characterization of seven compounds including four new peptides, namely acrepeptins A‒D (1e4), along with previously reported destruxin B (5), guangomide A (6), and guangomide B (7). Their structures were elucidated by spectroscopic analysis and compared with literatures. Of these, acrepeptins A (1) and C (3) showed markedly inhibitory activities on nitric oxide production in lipopolysaccharide-activated microglial BV-2 cells with IC50 values of 12.0 ± 2.3 and 10.6 ± 4.0 mM, respectively. Furthermore, acrepeptins A (1) and C (3) significantly attenuated the expression of inducible nitric oxide synthase in a concentration-dependent manner (5e40 mM).

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Recommended Citation

Hsiao, George; Wang, Shih-Wei; Chiang, Yin-Ru; Chi, Wei-Chiung; Kuo, Yueh-Hsiung; Phong, Do Anh; Chen, Chia-Yu; and Lee, Tzong-Huei (2020) "Anti-inflammatory effects of peptides from a marine algicolous fungus Acremonium sp. NTU492 in BV-2 microglial cells," Journal of Food and Drug Analysis: Vol. 28 : Iss. 2 , Article 9.
Available at: https://doi.org/10.38212/2224-6614.1062

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Publisher Note

Due to printer error, this article was previously made available with incorrect page numbers. The article has been repaginated and the article is now available with the correct page numbers for volume 28, issue 2 283-291.