A mechanistic and empirical review of antcins, a new class of phytosterols of formosan fungi origin

Authors

Kumar K.J. Senthil, National Chung Hsing University
Vani M. Gokila, National Chung Hsing University
Chieh-Yin Chen, National Taiwan University
Wen-Wei Hsiao, National Taiwan University
Jing Li, ujian Agriculture and Forestry University
Zhan-xi Lin, Fujian Agriculture and Forestry University
Fang-Hua Chu, National Taiwan University
Gow-Chin Yen, National Chung Hsing UniversityFollow
Sheng-Yang Wang, Academia Sinica

Abstract

Tumor cell-induced platelet aggregation (TCIPA) is a mechanism that involves the protection of tumor cells in the circulation and the promotion of tumor cell invasion and metastases. The C-type lectin-like receptor 2 (CLEC-2) that binds podoplanin (PDPN) is on the platelet surface and facilitates the TCIPA. Selective blockage of the PDPN-mediated platelet-tumor cell interaction is thereby a plausible strategy for inhibiting metastases. In a search for antagonists of PDPN- and tumor cell-induced platelet aggregation, traditional Chinese medicines were screened and it was found that the water extract of Artemisia argyi leaves selectively inhibited the PDPN-induced platelet aggregation. Bioactivity-guided fractionation analysis was performed for defining a polysaccharide-containing fraction (AAWAP) characterized by inhibition of PDPN activity and tumor cell-induced platelet aggregation. The pharmacological effects of AAWAP on PDPN-activated CLEC-2 signaling were determined by using Western blot and alpha screening analyses. AAWAP was non-toxic to the cells and platelets and it suppressed PDPN- and tumor cell-induced platelet aggregation by irreversibly blocking the interaction between PDPN and CLEC-2 in a dose-dependent manner. These findings indicate that AAWAP is an antagonist of the PDPN-CLEC-2 interaction. This action by AAWAP may result in the prevention of tumor cell metastases, and if so, could become an effective pharmacological agent in treating cancer patients. © 2019

Abstract Image

ScienceDirect Link

10.1016/j.jfda.2019.09.001

Recommended Citation

Senthil, Kumar K.J.; Gokila, Vani M.; Chen, Chieh-Yin; Hsiao, Wen-Wei; Li, Jing; Lin, Zhan-xi; Chu, Fang-Hua; Yen, Gow-Chin; and Wang, Sheng-Yang (2020) "A mechanistic and empirical review of antcins, a new class of phytosterols of formosan fungi origin," Journal of Food and Drug Analysis: Vol. 28 : Iss. 1 , Article 3.
Available at: https://doi.org/10.1016/j.jfda.2019.09.001

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https://www.sciencedirect.com/sdfe/reader/pii/S1021949819300857/pdf