Article Title
WITHDRAWN: Paper based electrochemical biosensor using haemoglobin nanoparticles for detection of acrylamide in processed foods
Abstract
We describe herein the construction of an electrochemical paper based device (EPAD) for detection of acrylamide, a carcinogen and neurotoxin generated during thermal processing of foods through Maillard reaction. To achieve it, haemoglobin (Hb) nanoparticles (NPs) were prepared by desolvation method and characterized by transmission electron microscopy, UV–visible spectroscopy, X-ray diffraction and atomic force microscopy (AFM). The HbNPs were drop casted onto PAD for electrochemical detection of acrylamide. The EPAD showed the optimum response within 2 s at pH 5.5 and 20 °C. The working range of EPAD was 0.1 nM–100 mM with a limit of detection (LOD) of 0.1 nM. Analytical recovery of added acrylamide in food samples, at a final concentration of 15 and 20 mM, was 98.67% & 99% and within and between coefficient of variation (CV) 3.65% and 4.35% respectively, as measured by present EPAD. The EPAD was employed for detection of acrylamide in various processed foods. © 2018
