Determination of urea in milk based on N-bromosuccinimide–dichlorofluorescein postchemiluminescence method

Authors

F. Nie
N. Wang
P. Xu
J. Zheng

Abstract

A new postchemiluminescence (PCL) reaction was observed when urea was injected into the reaction mixture after the CL reaction of N-bromosuccinimide and dichlorofluorescein. A possible reaction mechanism was proposed based on the studies of the CL kinetic characteristics, CL spectra, fluorescence spectra, and other experiments. A new flow injection CL method for the determination of urea was established based on the PCL reaction. The relative standard deviation for the determination of urea was 1.3% (n = 11, c = 5.0 × 10−8 g/mL). The CL intensity responded linearly to the concentration of urea in the range 2.0 × 10−9–1.0 × 10−6 g/mL (r = 0.9992). The detection limit was 7 × 10−10 g/mL. The method had been applied to the determination of urea in milk with satisfactory results. © 2016

Abstract Image

ScienceDirect Link

10.1016/j.jfda.2016.05.009

Recommended Citation

Nie, F.; Wang, N.; Xu, P.; and Zheng, J. (2017) "Determination of urea in milk based on N-bromosuccinimide–dichlorofluorescein postchemiluminescence method," Journal of Food and Drug Analysis: Vol. 25 : Iss. 3 , Article 31.
Available at: https://doi.org/10.1016/j.jfda.2016.05.009

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