Antioxidant activity and protective effects of cocoa and kola nut mistletoe (Globimetula cupulata) against ischemia/reperfusion injury in Langendorff-perfused rat hearts
Protection against cardiomyocyte damage following ischemia/reperfusion (I/R) injury is highly desirable in patients with ischemic heart disease. Hydromethanol extracts of Globimetula cupulata (mistletoe) growing on cocoa (CGCE) and kola nut (KGCE) trees were assessed for antioxidant content and cardioprotective potential against I/R. Graded concentrations (1-50 μg/mL) of CGCE or KGCE were tested on Langendorff-perfused rat hearts to evaluate the effects on the flow rate, heart rate, and force of cardiac contraction, while another set of hearts were subjected to biochemical analyses. Both extracts showed good antioxidant content and activity, but KGCE (EC50: 24.8±1.8 μg/mL) showed higher hydroxyl radical scavenging activity than CGCE (70.2±4.5 μg/mL). Both extracts at 3 μg/mL reversed (p < 0.001) membrane peroxidation and the significant decrease in nitrite level, coronary flow rate, and superoxide dismutase and catalase activity caused by the I/R cycle. It is concluded that G. cupulata protects against ischemia-reperfusion injury in rat hearts via augmenting endogenous antioxidants and significant restoration of altered hemodynamic parameters. Copyright © 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
Akinmoladun, A.C.; Olowe, J.A.; Komolafe, K.; Ogundele, J.; and Olaleye, M.T.
"Antioxidant activity and protective effects of cocoa and kola nut mistletoe (Globimetula cupulata) against ischemia/reperfusion injury in Langendorff-perfused rat hearts,"
Journal of Food and Drug Analysis: Vol. 24
, Article 27.
Available at: https://doi.org/10.1016/j.jfda.2015.10.007
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