Abstract
A rapid and simple high-performance liquid chromatography–UV method was developed for the separation and quantification of salbutamol, ractopamine, and clenbuterol in pork. A mixture of acetonitrile–formic acid–ammonium acetate was used as the mobile phase to separate three β-agonists on a C18 column with gradient. The effects of the addition of formic acid and ammonium acetate to mobile phases on the separation of β-agonists were investigated. These additives can greatly improve the resolution and sensitivity. Under the optimized chromatographic condition, this separation does not need extra sample preparation. Complete baseline separation of three β-agonists was achieved in < 20 minutes; the linear range is 0.2–50 μg/L with a correlation coefficient R2 value of > 0.99. Excellent method reproducibility was found by intra- and interday precisions with a relative standard deviation of < 3%. The detection limit (S/N = 3) was found to be < 0.05 μg/L; this method can be used for routine screening of the β-agonist residues in foods of animal origin before being identified by confirmatory methods.
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Recommended Citation
Yan, K.; Zhang, H.; Hui, W.; Zhu, H.; Li, X.; Zhong, F.; Tong, X.; and Chen, C.
(2016)
"Rapid screening of toxic salbutamol, ractopamine, and clenbuterol in pork sample by high-performance liquid chromatography - UV method,"
Journal of Food and Drug Analysis: Vol. 24
:
Iss.
2
, Article 11.
Available at: https://doi.org/10.1016/j.jfda.2015.12.002
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