Evaluation of iron-binding activity of collagen peptides prepared from the scales of four cultivated fishes in Taiwan

C.-Y. Huang
C.-H. Wu
J.-I. Yang
Y.-H. Li
J.-M. Kuo

Abstract

Iron deficiency is one of the most concerning deficiency problems in the world. It may generate several adverse effects such as iron deficiency anemia (IDA) and reduced physical and intellectual working capacity. The aim of this study is to evaluate the Fe(II)-binding activity of collagen peptides from fishery by-products. Lates calcarifer, Mugil cephalus, Chanos chanos, and Oreochromis spp are four major cultivated fishes in Taiwan; thousands of scales of these fish are wasted without valuable utilization. In this study, scales of these fish were hydrolyzed by papain plus flavourzyme. Collagen peptides were obtained and compared for their Fe(II)-binding activity. Collagen peptides from Chanos chanos showed the highest Fe(II)-binding activity, followed by those from Lates calcarifer and Mugil cephalus; that from Oreochromis spp exhibited the lowest one. Fe(II)-binding activity of collagen peptides from fish scales was also confirmed with a dialysis method. Molecular weight (MW) distributions of the collagen peptides from scales of four fish are all < 10 kDa, and averaged 1.3 kDa. Hydrolysates of fish scales were further partially purified with ion exchange chromatography. Fractions having Fe(II)-binding activity were obtained and their activity compared. Data obtained showed that collagen peptides from fish scales did have Fe(II)-binding activity. This is the first observation elucidating fish scale collagen possessing this functionality. The results from this study also indicated that collagen peptides from fish scales could be applied in industry as a bioresource. © 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.

ScienceDirect Link

10.1016/j.jfda.2014.06.009

Recommended Citation

Huang, C.-Y.; Wu, C.-H.; Yang, J.-I.; Li, Y.-H.; and Kuo, J.-M. (2015) "Evaluation of iron-binding activity of collagen peptides prepared from the scales of four cultivated fishes in Taiwan," Journal of Food and Drug Analysis: Vol. 23 : Iss. 4 , Article 23.
Available at: https://doi.org/10.1016/j.jfda.2014.06.009