Determination of cholesterol and four phytosterols in foods without derivatization by gas chromatography-tandem mass spectrometry
In this study, a method for determination of cholesterol and four phytosterols by gas chromatography coupled with electron impact ionization mode-tandem mass spectrometry without derivatization in general food was developed. The sample was saponified with 7.5% KOH in methanol. After heating on hot plate and reflux for 60 minutes, the saponified portion was extracted with n-hexane/petroleum ether (50:50, v/v). The extracts were evaporated with rotary evaporator and then redissolved with tetrahydrofuran. The tetrahydrofuran layer was transferred into an injection vial and analyzed by gas chromatography on a 30 m VF-5 column. Limit of quantification was 2 mg/kg. Recoveries of cholesterol and four phytosterols from general food were between 91% and 100%. © 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
Chen, Y.-Z.; Kao, S.-Y.; Jian, H.-C.; Yu, Y.-M.; Li, J.-Y.; Wang, W.-H.; and Tsai, C.-W.
"Determination of cholesterol and four phytosterols in foods without derivatization by gas chromatography-tandem mass spectrometry,"
Journal of Food and Drug Analysis: Vol. 23
, Article 19.
Available at: https://doi.org/10.1016/j.jfda.2015.01.010
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