Facile method for determination of deoxycytidine kinase activity in biological milieus
A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism. Copyright © 2013, Food and Drug Administration, Taiwan.
Hao, W.-H.; Yang, L.-C.; Wang, J.-J.; Hsu, C.-S.; Chang, L.-C.; and Hsu, K.-Y.
"Facile method for determination of deoxycytidine kinase activity in biological milieus,"
Journal of Food and Drug Analysis: Vol. 22
, Article 10.
Available at: https://doi.org/10.1016/j.jfda.2013.09.008
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.