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Article Title

Methanolic root extract of Rauwolfia serpentina improves the glucose tolerance in Wister mice

Abstract

In the present study, acute toxicity of methanolic root extract (MREt) of Rauwolfia serpentina (10-250 mg/kg) was observed in terms of hypoglycemic activity and behavior pattern including sedation and mortality rate in different groups of normoglycemic wister mice (6/group). Orally administrated MREt (10 to 60 mg/kg) was found non-sedative and showed hypoglycemic activity by reducing the mean blood glucose level from 80 to 62 mg/dL after 30 min of administration while the doses (100-250 mg/kg) of same extract showed sedation and mortality rate from 17 to 100% within 4 h. The value of median lethal dose (LD 50) of MREt of R. serpentina was 141.25 mg/kg (log LD50 = 2.15 mg/kg) from graph plotted between log-doses verses probits. In oral glucose tolerance test, MREt (10, 30 and 60 mg/kg) showed significant hypoglycemic activity at 0, 30 and 60 min by deceasing blood glucose level ranging from 31-65%, 39-49% and 25-51% respectively compared to control and negative control (treated with 0.05% dimethylsulfoxide @ 1 mL/kg) groups. However, MREt (100 mg/kg), though hypoglycemic from 0 to 120 min, induced slight sedation and mortality in experimental mice. The results indicated that the MREt (10-60 mg/kg) was non-sedative and effective in lowering the blood glucose level in mice but showed lethal effect in terms of sedation and mortality at doses ≥ 100 mg/kg. Therefore, MREt of R. serpentina in small doses can improve the glucose tolerance either at pancreatic or extra-pancreatic level.

ScienceDirect Link

10.6227/jfda.2012200208

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