A reversed-phase column liquid chromatographic method was developed for analysis of isosorbide dinitrate (ISDN), isosorbide 5-mononitrate (IS5MN) and isosorbide 2-mononitrate (IS2MN) and its pharmaceuticals. Chromatography is performed to Bondclone ODS column using a mobile phase consisting of acetonitrile-PIC B6 buffer, pH 4.75 (13:87) with ultraviolet wavelength at 220 nm. The linearity calibration range was 0.05 mg/ml to 2.5 mg/ml (r = 0.9999), and recoveries were generally greater than 98.0%
Tsai, F.-Y.; Chen, C.-J.; and Chien, C.-S.
"Determination of isosorbide nitrate and its analogues in pharmaceuticals by high-performance liquid chromatography,"
Journal of Food and Drug Analysis: Vol. 2
, Article 4.
Available at: https://doi.org/10.38212/2224-6614.3022