Simple, fast and very sensitive high performance liquid chromatography (HPLC) and capillary electrophoresis (CE) methods for the determination of rutin were developed. The separation of rutin by HPLC was performed using a Chromolith RP 18 column with detection wavelength at 356 nm. Composition of mobile phase was methanol : water (50 : 50, v/v) in 10 mM acetate buffer at pH 4.1. The limit of detection (at a signal-to-noise ratio of 3) was 23.91 ng/mL. Relative standard deviations of retention time, peak area and peak height (n = 5) for rutin were 0.199, 0.832 and 0.522%, respectively. For CE method, rutin was separated on a bare fused silica column. Borate buffer at pH 9.4 was used as the background electrolyte and detected wavelength was 208 nm. The limit of detection (at a signal-to-noise ratio of 3) was 55.67 ng/mL by using a 5 s injection time and +20 kV power supply. Relative standard deviations of retention time, peak area and peak height (n = 5) were 0.377, 0.923 and 2.446%, respectively. Both methods were applied to determine rutin in buckwheat tea and Fagopyrum tataricum seeds. Sample matrix in buckwheat tea was removed by using Sep-Pak C18 prior injection to HPLC and CE. The results for rutin determination obtained by the HPLC method agreed well with those from CE method.
Vachirapatama, N.; Chamnankid, B.; and Kachonpadungkitti, Y.
"Determination of rutin in buckwheat tea and Fagopyrum tataricum seeds by high performance liquid chromatography and capillary electrophoresis,"
Journal of Food and Drug Analysis: Vol. 19
, Article 18.
Available at: https://doi.org/10.38212/2224-6614.2214