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Article Title

Analysis of bioactive flavonoid-O-glycosides in Saussurea mongolica Franch by capillary zone electrophoresis using ionic liquids as the additive

Abstract

In this study, a capillary zone electrophoretic method, in which 1-alkyl-3-methylimidazolium-based ionic liquids (IL) was used as the additive, was established to separate and identify six bioactive flavonoid-O-glycosides in Saussurea mongolica Franch. In order to investigate the traits of IL in capillary electrophoresis (CE), the influences of the alkyl group, the anionic part and the concentration of IL were investigated and discussed. Optimum separation was achieved with 20 mM borate buffer at pH 9.00 containing 5 mg/mL 1-ethal-3-methylimidazolium tetrafluoroborate. The applied voltage was 15 kV and the capillary temperature was kept constant at 25°C. The relative standard deviations (RSD) of the migration time and the peak area of each peak for six replicate injections were 0.75%-1.45% and 3.18%-3.91%, respectively. Moreover, it was worth noticing that the mechanism of interaction between IL and analytes was investigated preliminarily.

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