A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based method has been developed and validated to confirm the presence of the synthetic growth promoter 17α-methyltestosterone (MT) in bovine serum samples. After a single-step extraction with acetonitrile, the enhanced determination of methyltestosterone was achieved by detecting its oxime derivative. In accordance with the criteria defined in Commission Decision 2002/657/EC and using deuterated testosterone (T-d3) as an internal standard, the method has been validated in terms of decision limit (0.05 ng/mL), detection capability (0.09 ng/mL), repeatability CV (7.04%), within-laboratory reproducibility CV (11.00%), and accuracy (87.00%).
Patricia, R.; Carolina, N.; Vázquez, B.I.; Alberto, C.; and Fente, C.A.
"Determination of 17α-methyltestosterone in bovine serum using liquid chromatography tandem mass spectrometry,"
Journal of Food and Drug Analysis: Vol. 18
, Article 7.
Available at: https://doi.org/10.38212/2224-6614.2224