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Article Title

Metabonomic study with a high performance liquid chromatography coupling to a triple quadruple mass spectrometer to identify biomarkers from urine of high-fat fed and streptozotocin treated rats

Abstract

High performance liquid chromatography coupled with an electrospray ionization (ESI) tandem mass (MS/MS) detector was employed to scan the urinary metabolites of Wistar rats (n=5 each group) fed with either control diet or high fat diet with a single dose of streptozotocin (STZ) for 14 weeks. Principle component analysis (PCA) of the LC-MS data was performed for pattern re-cognition and it clearly showed that the dosed animals displayed different metabolic responses even though they received the same treatment. Components responsible for the separation were identified with MS/MS. Glucose and malonic acid were among some other components in Krebs cycle to show high loading strength in PCA. It was therefore postulated that the high fat diets have driven the animals toward the metabolic flux to gluconeogenesis and then energy production through Krebs cycle. Excess energy was also stored through fatty acid biosynthesis with the observation of high loading strength of malonic acid. This experiment demonstrated that a triple quadruple mass spectrophotometer is capable of the metabonomic study on diet modulation of animals. Since metabonomics is a congruous part of nutrigenomics that can provide information on nutritional status, toxicity and gene function of an organism, it is hoped that this technique be extended to study the efficacy of functional foods and the development of personalized foods.

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