Article Title
Analysis of bioactive triterpenes in Eriobotrya japonica Lindl. by high-performance liquid chromatography
Abstract
Eriobotrya japonica (Rosaceae) has been used for clearing away heat in the lung to stop cough and lowering the adverse flow of gi to stop vomiting. High-performance liquid chromatographic method was applied to assay the five triterpenes contents in E. japonica from various locations. Samples were analyzed on a HyPURITY C-18 column (5 μm, 4.6 × 250 mm) eluted at a rate of 0.5 mL/min with a solvent of A - B (A, Methanol; B, 0.15% acetic acid aqueous; A: B = 85: 15, v/v). Triterpenes were detected by refractive index detection (RID). Regression equations of the calibration curves revealed good linear relationships (correlation coefficients: 0.9998-1.0000). The recoveries ranged between 98.39% - 102.41% and indicated good accuracy.
Recommended Citation
Ho, H.-Y.; Lin, W.-C.; Kitanaka, S.; Chang, C.-T.; and Wu, J.-B.
(2008)
"Analysis of bioactive triterpenes in Eriobotrya japonica Lindl. by high-performance liquid chromatography,"
Journal of Food and Drug Analysis: Vol. 16
:
Iss.
6
, Article 5.
Available at: https://doi.org/10.38212/2224-6614.2314