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Article Title

Use of solid phase extraction for sample clean-up and preconcentration of vitamin B12 in multivitamin tablet before HPLC-UV, UV and atomic absorption spectrophotometry

Abstract

A solid phase extraction method for sample clean-up and preconcentration of vitamin B12 in multivitamin tablet and its determination by HPLC-UV, UV and atomic absorption spectrophotometry is reported. Solid phase parameters such as maximum loading capacity and breakthrough volume were 400 μg and 1750 mL of 0.001 ppm for this vitamin respectively. A proper mobile phase used for HPLC was water/acetonitrile (75:25, v/v) with pH = 3.5 (adjusted with acetate buffer) at 25°C. Limit of detection and linear dynamic range for HPLC, UV and atomic absorption were 0.1, 0.1-25.0; 0.4, 0.4 -15.0; 2.7, 2.7-300.0 (μg/mL) respectively. Recovery of vitamin B12 was about 96%.

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