Collaborative study on the determination of 7-aminoflunitrazepam in urine by GC-MS
A previously published gas chromatography-mass spectrometry (GC-MS) method for the analysis of 7-aminoflunitrazepam (7-amino-FM2) in urine was subjected to an inter-laboratory collaborative study. The method was rigorously defined in terms of performance requirements, yet allowed some degrees of flexibility to individual analysts. The collaborative study followed the guidelines provided by the Association of Official Analytical Chemists International (AOAC International). Each of nine drug-abuse urine testing laboratories in Taiwan participated in the study to analyze 3 samples in duplicate. The repeatability relative standard deviation (RSDr) and the reproducibility relative standard deviation (RSDR) of the measurements of 7-amino-FM2 in urine were determined to be less than 9.91% and 14.84%, respectively. The method showed acceptable reproducibility, as evidenced by HORRAT values of 0.6 to 0.7. The method was thus proposed to be used by the testing laboratories in Taiwan.
Hsu, M.-C.; Chan, K.H.; Chu, W.-L.; and Liu, R.H.
"Collaborative study on the determination of 7-aminoflunitrazepam in urine by GC-MS,"
Journal of Food and Drug Analysis: Vol. 15
, Article 13.
Available at: https://doi.org/10.38212/2224-6614.2432