A new detection technique for fluoroquinolone-conjugated proteins by high performance liquid chromatography with UV/fluorescence detectors
This study was aimed to develop a simple, fast, and reliable technique to detect fluoroquinolones (FQs) -conjugated bovine serum albumin (BSA). Four tested FQs, enrofloxacin, ofloxacin, danofloxacin, and orbifloxacin, were conjugated with BSA by following the N-hydroxysuccinimide ester method. The technique was designed according to the different absorption characteristics of the FQs and BSA; FQs can be detected by both UV and fluorescence detectors, but BSA can only be detected by UV. The results demonstrated that the developed method was efficient in detecting FQs-BSA conjugates. In addition, the method not only trace the FQs and BSA conjugation responses but also can be used to estimate the level of FQs-BSA conjugation. Therefore, this technique is a valuable tool for the detection drug-carrier-conjugated antigens, especially for FQs-BSA-conjugates during the production of anti-FQs monoclonal antibodies.
Hung, S.-W.; Shih, C.-W.; Chen, B.-R.; Tu, C.-Y.; Ling, Y.-F.; Tsou, L.-T.; Ho, S.-P.; and Wang, W.-S.
"A new detection technique for fluoroquinolone-conjugated proteins by high performance liquid chromatography with UV/fluorescence detectors,"
Journal of Food and Drug Analysis: Vol. 15
, Article 13.
Available at: https://doi.org/10.38212/2224-6614.2448