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Article Title

Effect of melatonin on antioxidation in the PC12 cell line after exposure to ethanol

Abstract

Excessive ethanol consumption may increase the production of reactive oxygen species (ROS), which results in the oxidative damage of tissues. The purpose of this study was to evaluate the effects of melatonin on the antioxidative status in the PC12 cell line after exposure to ethanol. In this study, the PC12 cells were treated with the different concentrations of melatonin for 24 hr. Subsequently, the cells were incubated with a fresh medium containing 150 mM of ethanol for 4 hr. To observe the oxidative damage and evaluate the antioxidative status after acute exposure to ethanol, cell viability, percentage of lactate dehydrogenase released (% LDH released), glutathione (GSH) level, activities of superoxide dismutase (SOD) and glutathione reductase (GRx), and concentrations of the protein carbonyls were assayed. The results showed that cell viability and GSH were increased, whereas the ethanol-induced cytotoxicity and protein carbonyl concentration decreased when the PC12 cells were treated with 10, 100 and 1000 μM melatonin. In contrary, there was no significant difference in the activity of SOD in the ethanol-treated group. In addition, there were no significant differences in the activity of GRx in the groups with the treatment of 10 and 100 μM melatonin; however, GRx activity decreased in the cells treated with 1000 μM melatonin. In conclusion, melatonin of moderate concentration may be a protective agent to alleviate acute ethanol-induced oxidative damage by the generation of GSH in the PC12 cell line.

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