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Article Title

Determination of cobalamin in chlorella food by cation exchange column and graphite furnace atomic absorption spectrometry

Abstract

A method for the rapid determination of cobalamin (Vitamin B12) in chlorella food was proposed. This method utilized a strong cation exchange column to remove free cobalt (II), and the cobalamins were quantitatively determined by measuring total cobalt in the column effluent with graphite furnace atomic absorption spectrometry (GFAAS). Under optimized conditions, the method detection limit was 24 μg/100 g. The method was validated by spiking cobalamin standards in different chlorella food, recoveries from spike preparations ranged from 98.3 to 99.5%, with coefficients of variation of 1.2-4.9%. The results show the suitability of the proposed method for determining vitamin B12 in chlorella food.

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