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Article Title

Determination of seven pueraria constituents by high performance liquid chromatography and capillary electrophoresis

Abstract

Puerariae Radix is a commonly used Chinese herbal medicine derived from the dried root of the legume plant, which contains a series of isoflavones as its chief pharmacologically active constituents. Using 7 pueraria constituents as marker substances, a high performance liquid chromatography (HPLC and a capillary electrophoresis (CE) method were developed to assay the quality of Puerariae Radix within 65 and 50 min, respectively. Extracted samples were analyzed with a Cosmosil 5C18-MS column and eluted with a mixture of potassium dihydrogenphosphate (30 mM, adjusted to pH 3.0 with phosphoric acid) and aqueous acetonitrile solution, or an uncoated capillary (75 μm I.D.) and carried with an aqueous solution containing 10 mM sodium dihydrogenphosphate and 40 mM sodium dodecyl sulphate. The relative standard deviation of the marker substances, on the basis of peak-area ratios for 6 replicate injections, was 0.50∼0.97% (intraday) and 0.48∼0.97% (interday) for HPLC, and 0.88∼1.25% (intraday) and 0.85∼1.38% (interday) for CE. Both methods yielded accurate results differing by only 5% when applied to the analysis of practical samples of Puerariae Radix.

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