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Article Title

Determination of adenosine, cordycepin and ergosterol contents in cultivated Antrodia camphorata by HPLC method

Abstract

The concentrations of adenosine and cordycepin, 3'-deoxyadenosine in the hot water extract and ergosterol in the ethanol extract of a cultivated Antrodia camphorata were measured by high performance liquid chromatography (HPLC) method. The procedure were carried out on a reversed-phase C18 column by linear gradient elution, using four eluents as mobile phase and by isocratic elution, using 100% methanol as mobile phase, respectively. The analysis was completed within 20 min. The application of this method for quantitation of adenosine and cordycepin in 5-L and 100-L fermentation from two A.camphorata strains BCRC 35396 and BCRC 35398 were described. Comparison of adenosine, cordycepin and ergosterol contents were made with different treatment of 700-L fermentation broth.

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