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Abstract

On May 15, 2002, a food poisoning case caused by consumption of unknown gastropods was reported. Symptoms included vomiting, diarrhea and other neurological symptoms developed 2 hr after ingestion of gastropods. The unknown species of gastropod were later identified to be Nassarius Papillosus Alectrion and Nassarius Gruneri Niotha. Using a tetrodotoxin bioassay, the two samples showed toxicity of 320 and 386 mouse units per gram (MU/g) in Nassarius Papillosus and Nassarius Gruneri. The result of high performance liquid chromatography (HPLC) on the purified toxin indicated that the causative toxic agents were tetrodotoxin and its derivative anhydrotetrodotoxin.

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