Garambullo (Myrtillocactus geometrizans) Reduces Cell Viability via Apoptosis and Inhibits Colony Formation in HT-29 and SW480 Colon Cancer Cell Lines.

Authors

• López-Alvarez Guadalupe Soledad, Center for Advanced Biomedical Research, Faculty of Medicine, Autonomous University of Queretaro. Santiago de Querétaro 76140, México
• Gutierrez-Silerio Gloria Yareli, Center for Advanced Biomedical Research, Faculty of Medicine, Autonomous University of Queretaro. Santiago de Querétaro 76140, México.
• García-Solís Pablo, Center for Advanced Biomedical Research, Faculty of Medicine, Autonomous University of Queretaro. Santiago de Querétaro 76140, México
• Morales-Bárcenas Rocio, Subdirección de Investigación Básica, Instituto Nacional de Cancerología. Mexico City, 14080, México.
• Sánchez-Pérez Yesennia, Subdirección de Investigación Básica, Instituto Nacional de Cancerología. Mexico City, 14080, México
• Hernández-Puga Ana Gabriela, Center for Advanced Biomedical Research, Faculty of Medicine, Autonomous University of Queretaro. Santiago de Querétaro 76140, México
• Vergara-Castañeda Haydé Azeneth, Center for Advanced Biomedical Research, Faculty of Medicine, Autonomous University of Queretaro. Santiago de Querétaro 76140, MéxicoFollow

Keywords

apoptosis, cell viability, colony formation assays, colorectalButyric acid, Colorectal cancer, fermented extract of garambullo.Garambullo, Molecular docking, Programmed cell death.

Abstract

Garambullo (Myrtillocactus geometrizans) is a Mexican fruit rich in bioactive phytochemicals. Its fermented extract (FEG), obtained after in vitro gastrointestinal digestion and fermentation, was evaluated in HT-29 and SW480 colorectal cancer cells. FEG reduced cell viability (IC50: 2985 and 3200 μg/mL, respectively) and induced apoptosis and necrosis. In 3D assays, FEG inhibited colony formation, with complete suppression in HT-29 cells. Molecular docking further suggested that butyric acid identified in FEG interacts with Caspase-3 and RIPK1. These findings support the antiproliferative and pro-death effects of FEG in colorectal cancer models.

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Recommended Citation

Soledad, López-Alvarez Guadalupe; Yareli, Gutierrez-Silerio Gloria; Pablo, García-Solís; Rocio, Morales-Bárcenas; Yesennia, Sánchez-Pérez; Gabriela, Hernández-Puga Ana; and Azeneth, Vergara-Castañeda Haydé (2026) "Garambullo (Myrtillocactus geometrizans) Reduces Cell Viability via Apoptosis and Inhibits Colony Formation in HT-29 and SW480 Colon Cancer Cell Lines.," Journal of Food and Drug Analysis: Vol. 34 : Iss. 3 , Article 8.
Available at: https://doi.org/10.38212/2224-6614.3599

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